A second person has now died following a three-vehicle collision on Highway 401 west of Tilbury.
The Ontario Provincial Police said officers were investigating a multi-vehicle collision on the highway between Gracey Side Road and Comber Side Road, when a second collision occurred involving two tractor-trailers and a pickup truck at around 10:40 a.m. on August 23.
The driver of a tractor-trailer involved in the second crash was pronounced dead at the scene.
The driver of the pickup truck was rushed to hospital and has now died as a result of his injuries.
The third driver involved in the crash was not injured.
The identities of the drivers have not been released.
The collision remains under investigation and anyone who may have witnessed the incident is asked to contact the OPP at 1-888-310-1122 or Crime Stoppers anonymously at 1-800-222- 8477 (TIPS).